PerkinElmer
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IL-2 (mouse) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Mouse Interleukin-2 (IL-2) Detection Kit is designed for detection and quantitation of mouse IL-2 in serum, buffered solution, or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

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部件号 产品尺寸
AL511C 500 assay points
AL511F 5,000 assay points
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概览

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

In the mouse, Interleukin 2 (IL2), formerly known as T-cell growth factor (TCGF), contains 149 amino acids in its mature form. In humans, this immunoregulatory lymphokine is produced by lectin- or antigen-activated T cells and capable of inducing the thymic expansion of recently activated antigen-specific T lymphocytes. However, since T-cell immunity could be elicited by various agents in the absence of IL2 in vivo, it is currently thought that the main non-redundant activity of this cytokine is the induction of the suppressor function of CD4+CD25+ regulatory T cells (Treg) in peripheral lymph nodes to ensure suppression of autoreactive T cells that escape negative selection. Moreover, recent findings suggest that IL2-mediated regulation of Treg cells is important in the prevention of type 1 diabetes and autoimmune disease.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

免责声明:  For research use only. Not for use in diagnostic procedures.

规格

检测目标 IL2
检测目标类 Cytokine
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Mouse
治疗领域 Inflammation
产品尺寸 5,000 assay points

资源,活动及更多信息

资源类型 文件名 文件格式
产品手册 Alpha product listing PDF  124 KB
海报 AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices PDF  273 KB
数据单表 Manual AlphaLISA Mouse IL-2 AL511 PDF  209 KB
活动 Society of Laboratory Automation & Screening (SLAS)