The AlphaLISA® immunoassay kit for human placental growth factor (PIGF) enables the quantitative determination of human PIGF (PGF) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
|AL3030HV||100 Assay Points|
|AL3030C||500 Assay Points|
|AL3030F||5,000 Assay Points|
AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Placental growth factor (PlGF) is a protein encoded by the PGF gene and is recognized as a member of the vascular endothelial growth factor sub-family. PlGF has been identified as a pro-angiogenic factor where it is primarily secreted in the placenta as a homodimer. Further, PlGF has been found in other tissues of the heart, lungs, and eyes to name a few. Studies using gene knockout models have demonstrated that PlGF plays a major role in the regulation of inflammatory processes associated with neo-angiogensis via PlGF/VEGFR-1. These studies identified relations between aberrant PlGF levels and atherosclerosis, hypertension, bone and collagen repair, and arthritis. This AlphaLISA kit allows for the detection and quantification of PlGF in human serum, plasma, CSF, cell culture media and cell lysates.
免责声明: For research use only. Not for use in diagnostic procedures.
|产品尺寸||5,000 Assay Points|
|产品手册||Alpha product listing||PDF 124 KB|
|操作手册||AlphaLISA Human PIGF Detection kit AL3030||PDF 566 KB|
|活动||Society of Laboratory Automation & Screening (SLAS)|