PerkinElmer
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SHP-2 (human) AlphaLISA Detection Kit, 500 assay points

The AlphaLISA® immunoassay kit for human SHP-2 enables the quantitative determination of human Src-homology domain-2 containing protein tyrosine phosphatase 2 (SHP-2) in buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

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部件号 产品尺寸
AL3074HV 100 Assay Points
AL3074C 500 Assay Points
AL3074F 5,000 Assay Points
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概览

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

SHP-2 is Src-Homology domain-2 containing protein tyrosine Phosphatase 2, also known as PTPN11, BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2, protein tyrosine phosphatase, non-receptor type 11. It is a tyrosine phosphatase which dephosphorylates tyrosine residues in proteins. It is widely expressed in most tissues and acts as signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Mutations in this gene have been linked f Noonan syndrome, LEOPARD syndrome and childhood leukemia. The association with breast cancer, leukaemia, lung cancer, liver cancer, gastric cancer, laryngeal cancer, oral cancer and other cancer types makes SHP-2 useful as a tumor biomarker. This kit has been designed for the detection of human SHP-2 in cell culture media and cell lysate.

免责声明:  For research use only. Not for use in diagnostic procedures.

规格

检测目标 SHP2
检测目标类 Protein
自动化兼容 Yes
检测方法 Alpha
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Human
治疗领域 Metabolic
产品尺寸 500 Assay Points

资源,活动及更多信息

资源类型 文件名 文件格式
产品手册 Alpha product listing PDF  124 KB
操作手册 Protocol for AlphaLISA SHP-2 detection assay PDF  423 KB