PerkinElmer
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Osteoprotegerin (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for total osteoprotegerin enables the quantitative determination of human osteoprotegerin in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
AL3010HV
100 Assay Points
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AL3010C
500 Assay Points
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AL3010F
5,000 Assay Points
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Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor, is a human protein encoded by the gene TNFRSF11B (tumor necrosis factor receptor superfamily member 11B). It is a cytokine decoy receptor for nuclear factor kappa B ligand receptor, and neutralizes its function in osteoclastogenesis. Osteoprotegerin-mediated pathways maintain the structure of healthy bone by inhibiting osteoclast activation. It is released as a disulfide-linked homodimer, though the monomeric form is also present in human serum. The OPG pathway poses a potential therapy for osteopenic disorders, such as osteoporosis, arthritis and hypercalcemia. This AlphaLISA kit will detect both the homodimer and the monomeric forms of osteoprotegerin.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

规格

检测目标 Osteoprotegerin
检测目标类 Protein
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Human
治疗领域 Inflammation
产品尺寸 500 Assay Points
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Total Osteoprotegerin (Human) AlphaLISA Detection Kit

This manual explains how to run the AlphaLISA no-wash human total osteoprotegerin detection assay.

PDF 570 KB