PerkinElmer
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AlphaLISA unmodified Histone H3 Lysine 4 (H3K4) Cellular Detection Kit, 500 Assay Points

This kit is designed to be used as a control for the AlphaLISA Epigenetic Cellular Detection Kits, through detection of unmodified Histone H3 Lysine 4 (H3K4) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

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部件号 产品尺寸
AL719HV 100 assay points
AL719C 500 assay points
AL719F 5,000 assay points
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概览

The AlphaLISA® Epigenetic Cellular Detection Kits enable rapid and direct detection of endogenous modification of epigenetic marks on histone H3. These no wash, all-in-one-well assays are optimized for simplicity and throughput.

  • Simple assay protocol amenable to automation, eliminating tedious ELISAs and Westerns
  • Suitable for endogenous and recombinant cell lines, providing the flexibility to work with relevant cell models
  • Ideal for HTS protocols with demonstrated Z’ factors greater than 0.6

The AlphaLISA detection of epigenetic marks in cellular extracts is performed as follows: cell cultured in the presence of compounds are lysed with the Cell-Histone Lysis buffer. Histones are then extracted from the nucleosomes by the addition of the Cell-Histone Extraction buffer. AlphaLISA anti-mark Acceptor beads and Biotinylated anti-Histone H3 (C-terminus) antibodies are then added for the capture of histone proteins carrying the mark of interest. After incubation, Streptavidin Donor beads are added for the capture of the biotinylated antibody. In the presence of histone proteins bearing the mark of interest, the beads come into proximity. Excitation of the Donor beads provokes the release of singlet oxygen molecules that trigger a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

免责声明:  For research use only. Not for use in diagnostic procedures.

规格

检测目标 Histone H3
检测目标类 Histone
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
分子修饰 Unmodified
产品品牌名称 AlphaLISA
运输条件 蓝冰
治疗领域 Epigenetics
产品尺寸 500 assay points

资源,活动及更多信息

资源类型 文件名 文件格式
产品手册 Alpha product listing PDF  128 KB
海报 Comparison of endogenous methyl marks on histone H3 in cancer-derived cell lines using AlphaLISA PDF  1 MB
活动 Society of Laboratory Automation & Screening (SLAS)