PerkinElmer
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Anti-FLAG AlphaPlex-645 Acceptor beads, 25 mg

AlphaPlex-645 (Samarium) Acceptor beads conjugated to anti-FLAG antibody, for use in Alpha no-wash multiplexing assays.

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For research use only. Not for use in diagnostic procedures.

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AP112SM-C
250 µg
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AP112SM-M
5 mg
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AP112SM-R
25 mg
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详情 信息

AlphaPlex-645 (Samarium) Acceptor beads conjugated to anti-FLAG antibody. The mouse monoclonal M2 clone recognizes the FLAG sequence at the N-terminus, Met N-terminus, C-terminus, and internal sites. These beads can be used to capture FLAG-tagged proteins and peptides, and can be used with Alpha Donor beads to create AlphaPlex no-wash assays for:

  • Protein-protein interactions
  • Protein detection
  • Enzymatic assays

AlphaPlex Acceptor beads are intended to be used in multiplexing assays, in conjunction with AlphaLISA Acceptor beads and Alpha Donor beads. In a typical Alpha assay, 250 µg of Acceptor beads is sufficient to run 250-500 wells using a 50 µL reaction volume.

规格

抗体偶联物 Anti-FLAG
自动化兼容 Yes
珠型或核心珠型 AlphaPlex Samarium (645) Acceptor
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaPlex
包装量 1.0 Units
运输条件 蓝冰
产品尺寸 25 mg
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指南

Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.

PDF 380 KB
User's Guide To Alpha Assays Protein:Protein Interactions

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.

PDF 3 MB