PerkinElmer
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AlphaScreen FLAG (M2) Detection Kit, 500 assay points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-FLAG conjugated AlphaScreen Acceptor beads. 

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
6760613C
500 assay points
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6760613M
10,000 assay points
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6760613R
50,000 assay points
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详情 信息

This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume. The M2 antibody clone can recognize N-terminal, C-terminal, and internal FLAG tags.

This bead kit can be used for many applications, including:

  • Protein-protein interaction assays
  • Protein-peptide interaction assays
  • FLAG-tagged protein detection and quantitation
  • Protein expression
  • Binding studies

In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is FLAG-tagged, and associates with the AlphaScreen anti-FLAG Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated FLAG probe provided in the kit.

规格

抗体偶联物 Streptavidin/Anti-FLAG
自动化兼容 Yes
产品品牌名称 AlphaScreen
运输条件 蓝冰
产品尺寸 500 assay points
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产品手册

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 4 MB

指南

Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.

PDF 380 KB