PerkinElmer
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AlphaScreen Histidine (Nickel Chelate) Detection Kit, 10,000 assay points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and nickel chelate (Ni-NTA) AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
6760619C
500 assay points
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6760619M
10,000 assay points
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6760619R
50,000 assay points
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詳情 信息

This bead kit can be used for many applications, including:

  • Protein-protein interaction assays
  • Protein-peptide interaction assays
  • His-tagged protein detection and quantitation
  • Protein expression
  • Binding studies

In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is His-tagged, and associates with the AlphaScreen nickel chelate Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated His peptide probe provided in the kit.

规格

抗体偶联物 Streptavidin/Nickel chelate
自动化兼容 Yes
产品品牌名称 AlphaScreen
包装量 10000.0 Units
运输条件 蓝冰
产品尺寸 10,000 assay points