AlphaLISA® Acceptor beads conjugated to anti-sheep IgG antibody. The antibody is a rabbit polyclonal antibody that is Fc-specific. These beads can be used to capture sheep IgG antibodies for AlphaLISA no-wash assays.
These beads can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash immunoassays for:
In a typical AlphaLISA assay, 1 mg of Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL reaction volume.
AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym ""Alpha"" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.
免责声明: For research use only. Not for use in diagnostic procedures.
|指南||Alpha Protein-Protein Interaction Quick Start Guide||PDF 380 KB|
|产品手册||Alpha product listing||PDF 124 KB|
|指南||AlphaLISA immunoassay development quick guide||PDF 1 MB|
|应用文献||Biochemical Binding ADCC Assays Utilizing AlphaLISA Toolbox Reagents||PDF 1 MB|
|技术说明||Designing Your Own AlphaLISA Assay: Selecting Toolbox Bead Pairs to Avoid Potential Bead-Bead Intera||PDF 1 MB|
|应用文献||Developing Novel Tetraubiquitin Substrates and AlphaLISA Technology to Provide Next Generation Deubi||PDF 2 MB|
|应用文献||Development and Utilization of Bromodomain and Extra Terminal Domain (BET) Assays Using AlphaLISA||PDF 1 MB|
|应用文献||Development of Pharmacokinetic (PK) Assays for Detecting Biosimilars Targeting TNFa Using AlphaLISA||PDF 2 MB|
|应用文献||Development of an AlphaLISA Assay for Screening Inhibitors of the TIM-3/Gal-9 Interaction||PDF 3 MB|
|指南||ELISA to AlphaLISA Immunoassay Conversion Guide||PDF 1 MB|
|产品手册||Handle Large Biomolecular Interactions With Ease||PDF 798 KB|
|指南||User's Guide To Alpha Assays Protein:Protein Interactions||PDF 3 MB|
|活动||Society of Laboratory Automation & Screening (SLAS)|