PerkinElmer
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Anti-Sheep IgG AlphaLISA® Acceptor beads, 5 mg

AlphaLISA® Acceptor beads conjugated to anti-sheep IgG antibody. The antibody is a rabbit polyclonal antibody that is Fc-specific. These beads can be used to capture sheep IgG antibodies for AlphaLISA no-wash assays.

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
AL132C
250 µg
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AL132M
5 mg
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AL132R
25 mg
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詳情 信息

These beads can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash immunoassays for:

  • Analyte detection assays
  • Biomarker detection assays
  • Antibody detection assays
  • Other immunoassays

In a typical AlphaLISA assay, 1 mg of Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL reaction volume.

Features:

  • No-wash steps, no separation steps
  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym ""Alpha"" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

规格

抗体偶联物 Anti-sheep IgG
自动化兼容 Yes
珠型或核心珠型 AlphaLISA Acceptor
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
产品尺寸 5 mg