PerkinElmer
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Biotin-11-ddCTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.

部件号 NEL546001EA
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详情 信息

Biotin labeled dideoxycytosine triphosphate (ddCTP) is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations. Biotin-labeled products may be detected with streptavidin conjugates. For the highest sensitivity in detection, we recommend using a streptavidin-HRP conjugate with tyramide signal amplification (TSA).

The number “11” in the compound name refers to the length of the linker arm between ddCTP and the label.

规格

半抗原 Biotin
核苷酸 ddCTP
运输条件 环境
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应用文献

Signal Amplification Strategies for Rapid and Sensitive High Content Analysis

TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.

PDF 776 KB

数据单表

NEL546 - BIOTIN-11-ddCTP - Technical Data Sheet

Nucleotide analogs are biologically active with a variety of DNA and/or RNA polymerases. Labeling methods such as: nick translation, random priming, polymerase chain reaction, 3'-end labeling, or transcription of RNA using SP6, T3, or T7 RNA polymerases may be used.

PDF 55 KB