PerkinElmer
check quantity

CXCL10a (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human CXCL10 Detection Kit is designed for detection and quantitation of human CXCL10 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

项目已成功添加到购物车

For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
TRF1259C
500 Assay Points
更多
TRF1259M
10,000 Assay Points
更多
立即购买

请输入有效的数量

请登录后再添加至收藏夹

购物车为空

详情 信息

The LANCE® Ultra Human CXCL10 (IP-10) Detection Kit is designed for detection and quantitation of human CXCL10 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

C-X-C Motif Chemokine 10 (CXCL10), also known as 10 kDa Interferon-gamma-Induced Protein (IP-10), is a 77 amino acid protein secreted by several cell types in response to IFN-γ. These cell types include monocytes, endothelial cells, and fibroblasts. CXCL10 has been attributed several roles such as chemoattraction for monocytes, T cells, and dendritic cells. Other activities include promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation. It has been suggested that CXCL10 may play an important role in delayed hypersensitivity reactions. Increased levels of CXCL10 are found in psoriatic plaques characterized by the infiltration of neutrophils, but it does not activate neutrophils. It was reported that CXCL10 also possesses antimicrobial activity. Recent data suggest that CXCL10 could bind to toll-like receptor 4 and may contribute to beta cell failure in diabetes.

规格

检测目标 CXCL10
检测目标类 Cytokine
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
实验类型 In vitro
产品品牌名称 LANCE Ultra
运输条件 蓝冰
Target Species Human
治疗领域 Inflammation
产品尺寸 10,000 Assay Points