Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
This Cyanine 5 labeled analog of deoxyuridine triphosphate (dUTP) is useful for labeling with a variety of methods including nick translation, random priming, polymerase chain reaction (PCR), and 3'-end labeling. Labeled products may be detected directly via fluorescence.
Fluorescent nucleotide analogs are biologically active with a variety of DNA and/or RNA polymerases. Labeling methods such as: nick translation, random priming, polymerase chain reaction, 3'-end labeling, or transcription of RNA using SP6, T3, or T7 RNA polymerases may be used.