Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
This Cyanine 5 labeled analog of uridine triphosphate (UTP) is used for labeling RNA probes via in vitro transcription with various RNA polymerases. Cyanine 5 labeled RNA probes are used in a variety of molecular biology applications including in situ hybridization and gene expression microarrays. Labeled products may be detected directly via fluorescence.
Fluorescent nucleotide analogs are biologically active with a variety of DNA and/or RNA polymerases. Labeling methods such as: nick translation, random priming, polymerase chain reaction, 3'-end labeling, or transcription of RNA using SP6, T3, or T7 RNA polymerases may be used.