PerkinElmer
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DELFIA Eu-N1 Rabbit Anti-Mouse-IgG antibody, 1 mg

Europium-labeled anti-mouse IgG antibody, for capturing antibodies in DELFIA® TRF assays.

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AD0124
50 µg
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AD0207
1 mg
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詳情 信息

Generic DELFIA reagents are intended for use in dissociation-enhanced time-resolved fluorometric assays. All reagents have been labeled using the DELFIA N1-chelate. They are suitable for highly sensitive endpoint measurements in separation based assays such as those for detection of protein-protein binding and cell adhesion as well as for quantitative immunoassays.

DELFIA (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology that provides a unique alternative to traditional ELISA. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA rabbit anti-mouse IgG antibody reagents are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of autofluorescent background.

The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to an increased signal-to-noise ratio. Sensitivity of the rabbit anti-mouse IgG antibody assay is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).

规格

抗体偶联物 Anti-mouse IgG
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), DELFIA TRF
实验类型 In vitro
荧光基团 Eu-N1
产品品牌名称 DELFIA
运输条件 蓝冰
产品尺寸 1 mg