PerkinElmer
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DELFIA Eu-N1-ITC Chelate and Eu Standard, 20 mg

Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.

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AD0001
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概览

DELFIA® Eu-N1 ITC chelate is optimized for the europium labeling of proteins and peptides for use in dissociation-enhanced time-resolved fluorometric assays.

Isothiocyanate (ITC) activated chelates react primarily with free amino groups on the compound to be labeled.

1 mg of this lyophilized preparation allows labeling of up to 5 mg of antibodies.

DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA assays are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of autofluorescent background. The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to increasing signal-to-noise ratio. Sensitivity is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).

规格

自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), DELFIA TRF
实验类型 In vitro
荧光基团 Eu-N1-ITC
产品品牌名称 DELFIA
运输条件 蓝冰
产品尺寸 20 mg

资源,活动及更多信息

资源类型 文件名 文件格式
操作手册 DELFIA Sm-Labelling kit 1244-303 PDF  83 KB
应用文献 Multiplexing DELFIA® assays using lanthanide-labeled probes PDF  121 KB
应用文献 Optimization and Miniaturization of DELFIA TRF Assays Converted from ELISA PDF  2 MB
应用文献 Simple Conversion of ELISA to DELFIA TRF Technology PDF  1 MB