PerkinElmer
check quantity

E.coli HCP LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra E. coli Host Cell Proteins Detection Kit is designed for detection and quantitation of E.coli HCP in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

项目已成功添加到购物车

For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
TRF1261C
500 Assay Points
更多
TRF1261M
10,000 Assay Points
更多
立即购买

请输入有效的数量

请登录后再添加至收藏夹

购物车为空

詳情 信息

The LANCE® Ultra E. coli HCP Detection Kit is designed for detection and quantitation of human E. coli host cell proteins in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Escherichia coli (E. coli) bacteria are commonly utilized as an expression system for the production of human recombinant proteins at industrial scale. However, since very frequently these proteins are intended to be used as therapeutics for humans, they must be subsequently purified to remove undesired contaminants that could elicit toxic or immunological reactions. Therefore, there is a current need to quantitate and trace these unwanted host cell proteins in protein preparations with excellent sensitivity and robustness, while maintaining high throughput capabilities.

规格

检测目标 E.coli HCP
检测目标类 Protein
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
产品品牌名称 LANCE Ultra
运输条件 蓝冰
治疗领域 Biologics/Bioprocess
产品尺寸 500 Assay Points