PerkinElmer
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Unconjugated AlphaLISA Acceptor beads, 1 mg

Unconjugated AlphaLISA Acceptor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own AlphaLISA Acceptor beads for use in Alpha no-wash assays.

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
6772001
1 mg
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6772002
5 mg
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6772003
50 mg
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详情 信息

AlphaLISA is a no-wash ELISA alternative and can be used for applications such as:

  • Analyte detection assays
  • Protein-protein interaction assays
  • Protein-DNA interaction assays
  • Protein-RNA interaction assays
  • Protein-small molecule interaction assays
  • Protein detection assays
  • Antibody detection assays
  • Antibody binding assays
  • Immunogenicity assays
  • Enzymatic assays

In a typical AlphaLISA assay, 1 mg of Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL reaction volume.

规格

抗体偶联物 Unconjugated
自动化兼容 Yes
珠型或核心珠型 AlphaLISA Acceptor
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
产品尺寸 1 mg
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产品手册

指南

Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.

PDF 380 KB
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

PDF 1 MB
User's Guide To Alpha Assays Protein:Protein Interactions

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.

PDF 3 MB