PerkinElmer
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LANCE Ultra Europium-anti-acetyl-p53 Lysine 382 (p53K382ac) Antibody, 10 µg

Europium-labeled monoclonal antibody recognizing human p53 acetylated at lysine 382 (p53K382ac). For use in LANCE® Ultra epigenetic writer and eraser assays.

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部件号
产品尺寸
TRF0409-D
10 µg
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TRF0409-M
100 µg
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详情 信息

The LANCE® Ultra Europium-anti-acetyl-p53 Lysine 382 (p53K382ac) antibody was used for the development and optimization of a SIRT1 deacetylase assay using a biotinylated p53 (368-393) peptide acetylated at lysine 382 as substrate. A technical note describing
the assay is available in our product literature.

Formats:

  • 10 µg = 1,500 assay points (384-well assay format)
  • 100 µg = 15,000 assay points (384-well format)

Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.

Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.

 

规格

抗体偶联物 Anti-p53K382ac
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
实验类型 In vitro
荧光基团 Eu-W1024
分子修饰 Acetylation
产品品牌名称 LANCE Ultra
运输条件 干冰
产品尺寸 10 µg
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