PerkinElmer
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LANCE Ultra Europium-anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) Antibody, 100 µg

Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 36 (H3K36me2). For use in LANCE® Ultra epigenetic writer and eraser assays.

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部件号
产品尺寸
TRF0408-D
10 µg
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TRF0408-M
100 µg
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详情 信息

The LANCE® Ultra Europium-anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) antibody was used for the development and optimization of a JMJD2a demethylase assay using a biotinylated Histone H3 (21-44) peptide tri-methylated at lysine 36 as substrate. A technical note describing
the assay is available in our product literature.

Formats:

  • 10 µg = 1,500 assay points (384-well assay format)
  • 100 µg = 15,000 assay points (384-well format)

Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.


Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.

规格

抗体偶联物 Anti-H3K36me2
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
实验类型 In vitro
荧光基团 Eu-W1024
分子修饰 Methylation
产品品牌名称 LANCE Ultra
运输条件 干冰
产品尺寸 100 µg
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