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LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36), 10 μg

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from IkappaB-alpha phosphorylated at Ser32/36. IkappaB-alpha (Ser32/36) peptides can be phosphorylated by the IKKβ kinase. Sequence motif recognized: RHDSGLDSM




部件号 产品尺寸
TRF0206-D 10 µg
TRF0206-M 100 µg



LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:

  • Test conditions: Rapid assay setup to move straight into optimized assay performance

  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing

  • Phospho-motifs: Substrate sequences are provided

  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For this antibody

  • 10 μg = 1,562 assay points

  • 100 μg = 15,625 assay points

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.


抗体偶联物 Anti-phospho-I?Ba
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
实验类型 In vitro
荧光基团 Eu-W1024
分子修饰 Phosphorylation
产品品牌名称 LANCE Ultra
运输条件 蓝冰
产品尺寸 10 µg