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DELFIA® Eu-Solution, 200mL (for Cytotoxicity Detection Only)

DELFIA® Europium solution for use in time-resolved fluorescence (TRF) cytotoxicity assays.

部件号 C135-100
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详情 信息

The DELFIA europium solution is intended for use in cell-mediated DELFIA cytotoxicity assay. It forms part of the AD0116 DELFIA® EuTDA Cytotoxicity Reagents pack, but is also available as an individual product. The individually available DELFIA Eu-solution is sufficient for 10x96 wells.

DELFIA cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.

规格

自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), DELFIA TRF
实验类型 In vitro
产品品牌名称 DELFIA
产品尺寸 200 mL
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应用文献

A Simplified, Gentle Cell-Labelling Method for Non-Radioactive Cytotoxicity Assays

Discover PerkinElmer's simplified, gentle cell-labelling method for non-radioactive cytotoxicity assays

PDF 497 KB