PerkinElmer
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Secretin AequoScreen Cell Line

Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.

部件号 ES-712-A
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This product contains living cells. Some cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.

詳情 信息

AequoScreen® Double Transfected Cell Line: Human recombinant Secretin receptor in aequorin CHO-K1 host cell. We provide two vials of cryopreserved cells (approximately 2.5 x 106 cells/vial), detailed product information including cell line properties, culture conditions and the pharmacological properties of the recombinant receptor in a functional assay. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some of our receptors may be restricted for sale in specified countries. Please inquire at your local sales office for more information.

Features:

  • Fully automatable and miniaturizable to 1536
  • Enables reduction in reagents and consumable costs
  • High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
  • Robust assay (Z', CV values, solvent resistance)
  • Reduces the number of false positives from autofluorescent compounds in screening libraries
  • Option to work with cells in suspension to increase throughput
  • Amenable for use with calcium coupled ion channels and tyrosine kinase receptors

The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

规格

检测目标类 GPCR
检测目标类型 Cell line
检测验证 Calcium Luminescence
G-Alpha天然受体 Gs
宿主细胞 CHO-K1
产品品牌名称 AequoScreen
受体类型 Glucagon
第二信使释放 Calcium flux
运输条件 干冰
治疗领域 Metabolic
产品尺寸 5 million cells