check quantity

GPR99 AequoScreen; cell line

Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.







AequoScreen® Double Transfected Cell Lines: GPR99 (Citric acid cycle). Human Recombinant, in CHO-K1 host cell. Two vials of cryopreserved cells are shipped per order. A detailed technical dossier includes sequence, culture conditions and pharmacological properties of the recombinant receptor. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some products are not available in some countries. Please inquire at your local sales office for more information.


  • Fully automatable and miniaturizable to 1536
  • Enables reduction in reagents and consumable costs
  • High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
  • Robust assay (Z', CV values, solvent resistance)
  • Reduces the number of false positives from autofluorescent compounds in screening libraries
  • Option to work with cells in suspension to increase throughput
  • Amenable for use with calcium coupled ion channels and tyrosine kinase receptors

The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

免责声明:  This product contains living cells. Some cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.


检测目标类 GPCR
检测目标类型 Cell line
检测验证 Calcium Luminescence
G-Alpha天然受体 Gi/Go
宿主细胞 CHO-K1
产品品牌名称 AequoScreen
受体类型 GPR
第二信使释放 Calcium flux
运输条件 干冰
产品尺寸 5 million cells


资源类型 文件名 文件格式
单页 Aequorin FLIPR PDF  1 MB
海报 Running an Aequorin Cell-Based Luminescent Assay on the FLIPR TETRA PDF  270 KB