PerkinElmer
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Serotonin 5-HT2C (edited) AequoScreen; cell line

Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.

部件号 ES-315-A
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This product contains living cells. Some cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.

详情 信息

AequoScreen® Double Transfected Cell Lines: Serotonin, 5-HT2C (edited) subtype. Human Recombinant, in CHO-K1 host cell. Two vials of cryopreserved cells are shipped per order. A detailed technical dossier includes sequence, culture conditions and pharmacological properties of the recombinant receptor. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some products are not available in some countries. Please inquire at your local sales office for more information.

Features:

  • Fully automatable and miniaturizable to 1536
  • Enables reduction in reagents and consumable costs
  • High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
  • Robust assay (Z', CV values, solvent resistance)
  • Reduces the number of false positives from autofluorescent compounds in screening libraries
  • Option to work with cells in suspension to increase throughput
  • Amenable for use with calcium coupled ion channels and tyrosine kinase receptors

The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

规格

检测目标类 GPCR
检测目标类型 Cell line
检测验证 Binding, Calcium Luminescence, Calcium Fluorescence
G-Alpha偶联蛋白 Ga16
G-Alpha天然受体 Gq/G11
宿主细胞 CHO-K1
产品品牌名称 AequoScreen
受体类型 5-Hydroxytryptamine (Serotonin)
第二信使释放 Calcium flux
运输条件 干冰
治疗领域 Central Nervous System, Cardiovascular, Metabolic, Gastrointestinal
产品尺寸 5 million cells
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产品手册

Take the Stress Out of Functional Testing - AequoZen and cAMPZen ready-to-use cells

PerkinElmer’s validated, ready-to-use AequoZen™or cAMPZen™ frozen, irradiated cells make it easier for you to perform functional testing of GPCRs. Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you get the testing done. So you can concentrate on results.

PDF 73 KB

技术说明

操作手册

AequoZen Frozen Cells Starter Kit

G protein coupled receptors (GPCRs) have been considered as a highly “druggable” target for many years, with over 40% of marketed drugs acting to modulate their function. For many years, radiometric techniques have dominated GPCR screening. However in the last decade the development of functional assays, where the effect of molecules is evaluated in terms of GPCR activation, has accelerated.In particular, measurement of calcium signaling and the development of molecular strategies which couple the majority of GPCRs to calcium signaling has allowed the use of high-throughput functional screening in GPCR research.

PDF 1 MB