Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.
Eu-W1024 labeled anti-6xHis antibodies for LANCE® assays.The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6X histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
|检测方法||Time-Resolved Fluorescence (TRF), TR-FRET|
Generic LANCE reagents are intended for setting up TR-FRET based assays using Eu-chelate label as a donor and APC-labeled reagent as an acceptor.