PerkinElmer
check quantity

LANCE Eu-W1024 Anti-phosphotyrosine (P-Tyr-100), 10µg

Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in LANCE® TR-FRET kinase assays.

项目已成功添加到购物车

部件号
产品尺寸
AD0203
10 µg
更多
AD0161
50 µg
更多
AD0162
1 mg
更多
立即购买

请输入有效的数量

请登录后再添加至收藏夹

购物车为空

详情 信息

Generic LANCE® reagents are intended for setting up homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) based assays using Eu-Chelate label as a donor and APC-labeled reagent as an acceptor. Generic reagents facilitate setting up LANCE® assays when there is a limited amount of specific reagents available or the assay reagents are relatively unstable. To enable optimization of both the signal or the noise ratio and signal stability, some of the generic reagents are labeled with two different chelates, W1024 or W8044.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

规格

抗体偶联物 Anti-P-Tyr-100
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
实验类型 In vitro
荧光基团 Eu-W1024
分子修饰 Phosphorylation
产品品牌名称 LANCE
运输条件 蓝冰
产品尺寸 10 µg
资源,活动及更多信息
  • 所有

产品手册

应用文献

Measurement of p38/MAPK Activity Using LANCE

Protein kinases regulate several important functions within cells including metabolism, cell cycle progression, angiogenesis, cell adhesion, etc. Specifically, mitogen-activated protein kinases (MAPK) play a central role in the cellular response to environmental stress, growth factors, and cytokines.

PDF 632 KB
Quench Correction for LANCE Time-Resolved Fluorescence Resonance Energy Transfer

This data sheet describes LANCETM Normalization using Wallac brand instruments. LANCE normalization can be performed either by using WIZARD software, which will guides us through the process or the Normalization samples which are run among other samples i

PDF 847 KB

操作手册

LANCE generic reagents

Generic LANCE reagents are intended for setting up TR-FRET based assays using Eu-chelate label as a donor and APC-labeled reagent as an acceptor.

PDF 101 KB