PerkinElmer
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LANCE Eu-W1024 Streptavidin, 1mg

Europium-labeled streptavidin, for capturing biotinylated biomolecules in LANCE TR-FRET assays.

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产品尺寸
AD0062
50 µg
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AD0063
1 mg
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詳情 信息

Isolated from fermentation filtrates of Streptomyces avidinii, 1 mg of streptavidin binds approximately 10 µg of biotin. The preparations are supplied ready to use in 50 mmol/L Tris-HCl buffered saline solution.

Generic LANCE® reagents are intended for setting up homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) based assays using Eu-Chelate label as a donor and APC-labeled reagent as an acceptor. Generic reagents facilitate setting up LANCE® assays when there is a limited amount of specific reagents available or the assay reagents are relatively unstable. To enable optimization of both the signal or the noise ratio and signal stability, some of the generic reagents are labeled with two different chelates, W1024 or W8044.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

规格

抗体偶联物 Streptavidin
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
实验类型 In vitro
荧光基团 Eu-W1024
产品品牌名称 LANCE
运输条件 蓝冰
产品尺寸 1 mg