The LANCE® Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).
|TRF0262||1,000 assay points|
|TRF0263||10,000 assay points|
|TRF0264||50,000 assay points|
PerkinElmer's LANCE® Ultra cAMP assay offers unmatched sensitivity and S/B using a simple TR-FRET protocol. Ideal for any Gs- or Gi-coupled GPCR assay, this Ultra-sensitive cAMP assay is supreme when working with low cell numbers, endogenous receptors, or difficult Gi-coupled receptors.
The LANCE Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).
The assay is based on the competition between the europium (Eu) chelate-labeled cAMP tracer and cellular cAMP for binding sites on cAMP-specific monoclonal antibodies labeled with the ULight™ dye. When antibodies are bound to the Eu-labeled cAMP tracer, light pulse at 320 or 340 nm excites the Eu chelate molecule of the tracer. The energy emitted by the excited Eu chelate is transferred by FRET to ULight molecules on the antibodies, which in turn emit light at 665 nm.
|检测方法||Time-Resolved Fluorescence (TRF), TR-FRET|
|产品尺寸||50,000 assay points|
|应用文献||APP_Phosphodiesterase4assayusingthenewLANCEUltracAMPkit.pdf||PDF 1 MB|
|海报||Automated 1,536-well cAMP Assay for the Identification and Characterization of Agonists and Antagonists of a Gi-coupled Receptor Using the New LANCE Ultra cAMP Kit.||PDF 408 KB|
|海报||High Sensitivity cAMP Assays in Primary Cells and Mesenchymal Stem Cells with the LANCE® Ultra cAMP kit||PDF 114 KB|
|应用文献||LANCE Ultra cAMP: A New TR-FRET cAMP Assay for Gs- and Gi-Coupled Receptors||PDF 862 KB|
|海报||LANCE Ultra cAMP: A New, Two-Component TR-FRET cAMP Assay for HTS of Gs- and Gi-Coupled Receptors||PDF 468 KB|
|产品手册||LANCE product listing||PDF 82 KB|
|指南||LANCE® Ultra cAMP Assay Development Guide||PDF 970 KB|
|操作手册||LANCE® Ultra cAMP Kit||PDF 707 KB|
|应用文献||Measuring Performance of an Automated and Miniaturized LANCE Ultra cAMP Assay for the Gi-coupled 5-HT1A Receptor- a Comparative Study||PDF 930 KB|