PerkinElmer
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LANCE Ultra cAMP Detection Kit, 50,000 assay points

The LANCE® Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).

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TRF0262
1,000 assay points
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TRF0263
10,000 assay points
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TRF0264
50,000 assay points
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详情 信息

PerkinElmer's LANCE® Ultra cAMP assay offers unmatched sensitivity and S/B using a simple TR-FRET protocol. Ideal for any Gs- or Gi-coupled GPCR assay, this Ultra-sensitive cAMP assay is supreme when working with low cell numbers, endogenous receptors, or difficult Gi-coupled receptors.

Features:

  • Unmatched assay sensitivity and signal stability-even with overnight incubation
  • Optimized screening of difficult targets and Gαi antagonists
  • Easier detection of cAMP response of endogenous receptors-use fewer cells per well
  • Robust sensitivity when miniaturized to 1536-well format for uHTS
  • Trusted results: stable pharmacology over time with consistent rank order potencies
  • Reproducible results with highest Z' values

The LANCE Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).

The assay is based on the competition between the europium (Eu) chelate-labeled cAMP tracer and cellular cAMP for binding sites on cAMP-specific monoclonal antibodies labeled with the ULight dye. When antibodies are bound to the Eu-labeled cAMP tracer, light pulse at 320 or 340 nm excites the Eu chelate molecule of the tracer. The energy emitted by the excited Eu chelate is transferred by FRET to ULight molecules on the antibodies, which in turn emit light at 665 nm.

规格

检测目标 cAMP
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
实验类型 In vitro
产品品牌名称 LANCE Ultra
第二信使释放 cAMP
运输条件 蓝冰
产品尺寸 50,000 assay points
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产品手册

应用文献

APP_Phosphodiesterase4assayusingthenewLANCEUltracAMPkit.pdf

Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of 3',5'-cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) to their corresponding 5’-nucleotide monophosphates, AMP and GMP.

PDF 1 MB
LANCE Ultra cAMP: A New TR-FRET cAMP Assay for Gs- and Gi-Coupled Receptors

Guanosine triphosphate binding protein coupled receptors (GPCRs) represent one of the largest and most important classes of pharmaceutical drug targets. Approximately 48% of all GPCRs couple through adenylate cyclase making the need for a robust cAMP detection method critical.

PDF 862 KB

指南

LANCE® Ultra cAMP Assay Development Guide

The LANCE® Ultra cAMP kit is intended for the quantitative determination of 3’,5’-cyclic adenosine monophosphate (cAMP) in cell lysate and cellular membrane samples.

PDF 970 KB

操作手册

海报

Automated 1,536-well cAMP Assay for the Identification and Characterization of Agonists and Antagonists of a Gi-coupled Receptor Using the New LANCE Ultra cAMP Kit.

The LANCE® Ultra cAMP assay is a second-generation LANCE time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by activated guanosine triphosphate binding protein-coupled receptors (GPCRs). The homogeneous two-component assay is based on the competition between europium chelate-labeled cAMP and cellular cAMP for binding to high-affinityanti-cAMP monoclonal antibodies labeled with the ULight™ dye.

PDF 408 KB
High Sensitivity cAMP Assays in Primary Cells and Mesenchymal Stem Cells with the LANCE® Ultra cAMP kit

Poster on High Sensitivity cAMP Assays in Primary Cells and Mesenchymal Stem Cells with the LANCE® Ultra cAMP kit

PDF 114 KB
LANCE Ultra cAMP: A New, Two-Component TR-FRET cAMP Assay for HTS of Gs- and Gi-Coupled Receptors

Guanosine triphosphate binding protein-coupled receptors (GPCRs) represent one of the largest and most important classes of pharmaceutical drug targets. We have developed a second generation LANCE® time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by activated GPCRs.

PDF 468 KB