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FSH (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human Follicle Stimulating Hormone (FSH) Detection Kit is designed for detection and quantitation of human FSH in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

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TRF1394C
500 Assay Points
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TRF1394M
10,000 Assay Points
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The LANCE® Ultra Human FSH Detection Kit is designed for detection and quantitation of human follicle stimulating hormone (FSH) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Follicle Stimulating Hormone (FSH) is a 35.5 kDa heterodimer (alpha and beta unit) glycoprotein that is synthesized by the anterior pituitary gland and regulates the fertility processes. The FSH molecule shares a common subunit (alpha chain) with Luteinizing Hormone (LH), Thyroid-Stimulating Hormone (TSH), and Human Chorionic Gonadotropin (hCG) while the beta subunit of FSH varies from LH, TSH, and hCH. The beta subunit is known to carry out specific biological activities on the ovary and testis. The level of FSH in serum is an excellent indicator for determining the status of oocyte maturation and follicle development in female. Infertility in females is often associated with mutations of the FSH gene resulting in reduced FSH synthesis. FSH is measured in International Units (IU). For recombinant FSH, one IU corresponds to approximately 0.065 to 0.075 µg of protein.

规格

检测目标 FSH
检测目标类 Hormone
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
实验类型 In vitro
产品品牌名称 LANCE Ultra
运输条件 蓝冰
Target Species Human
治疗领域 Metabolic
产品尺寸 10,000 Assay Points
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LANCE Ultra Human FSH Detection Kit TRF1394

This manual explains how to run a LANCE Ultra TR-FRET detection assay for human follicle stimulating

PDF 575 KB