The LANCE® Ultra Human Follicle Stimulating Hormone (FSH) Detection Kit is designed for detection and quantitation of human FSH in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
|TRF1394C||500 Assay Points|
|TRF1394M||10,000 Assay Points|
The LANCE® Ultra Human FSH Detection Kit is designed for detection and quantitation of human follicle stimulating hormone (FSH) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Follicle Stimulating Hormone (FSH) is a 35.5 kDa heterodimer (alpha and beta unit) glycoprotein that is synthesized by the anterior pituitary gland and regulates the fertility processes. The FSH molecule shares a common subunit (alpha chain) with Luteinizing Hormone (LH), Thyroid-Stimulating Hormone (TSH), and Human Chorionic Gonadotropin (hCG) while the beta subunit of FSH varies from LH, TSH, and hCH. The beta subunit is known to carry out specific biological activities on the ovary and testis. The level of FSH in serum is an excellent indicator for determining the status of oocyte maturation and follicle development in female. Infertility in females is often associated with mutations of the FSH gene resulting in reduced FSH synthesis. FSH is measured in International Units (IU). For recombinant FSH, one IU corresponds to approximately 0.065 to 0.075 µg of protein.
免责声明: For research use only. Not for use in diagnostic procedures.
|检测方法||Time-Resolved Fluorescence (TRF), TR-FRET|
|产品尺寸||10,000 Assay Points|
|单页||LANCE Ultra Biomarker Detection Kits||PDF 585 KB|
|操作手册||LANCE Ultra Human FSH Detection Kit TRF1394||PDF 575 KB|
|产品手册||LANCE product listing||PDF 82 KB|
|应用文献||Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET||PDF 3 MB|