PerkinElmer
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IL-6 (human) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra human IL-6 Detection Kit is designed for detection and quantitation of human interleukin-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
TRF1223C
500 Assay Points
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TRF1223M
10,000 Assay Points
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詳情 信息

The LANCE Ultra human IL-6 Detection Kit is designed for detection and quantitation of human interleukin-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Interleukin 6 (IL-6) is a ~22 kDa pleiotropic cytokine that acts not only on the immune system, but also affects many physiological events in various organs. IL6 exerts pro- or anti-inflammatory effects, depending on the target cell analyzed and the in vivo environmental circumstances. IL-6 is a differentiation and proliferation factor for B and T cells, and acts as a migration factor on monocytic cells. It is the major activator of acute-phase protein expression in the liver, a hematopoietic factor, and acts as a survival factor on neuronal cells. IL-6 signals through binding to the gp130/ IL-6R receptor complex, leading to the activation of JAK/STAT, MAPK and PI3K cascades.

规格

检测目标 IL6
检测目标类 Cytokine
自动化兼容 Yes
实验类型 In vitro
产品品牌名称 LANCE Ultra
运输条件 蓝冰
Target Species Human
治疗领域 Inflammation
产品尺寸 500 Assay Points