The LANCE® Ultra human IL-6 Detection Kit is designed for detection and quantitation of human interleukin-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
|TRF1223C||500 Assay Points|
|TRF1223M||10,000 Assay Points|
The LANCE Ultra human IL-6 Detection Kit is designed for detection and quantitation of human interleukin-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Interleukin 6 (IL-6) is a ~22 kDa pleiotropic cytokine that acts not only on the immune system, but also affects many physiological events in various organs. IL6 exerts pro- or anti-inflammatory effects, depending on the target cell analyzed and the in vivo environmental circumstances. IL-6 is a differentiation and proliferation factor for B and T cells, and acts as a migration factor on monocytic cells. It is the major activator of acute-phase protein expression in the liver, a hematopoietic factor, and acts as a survival factor on neuronal cells. IL-6 signals through binding to the gp130/ IL-6R receptor complex, leading to the activation of JAK/STAT, MAPK and PI3K cascades.
免责声明: For research use only. Not for use in diagnostic procedures.
|产品尺寸||500 Assay Points|
|技术说明||Comparison of LANCE Ultra and HTRF Assays for Measuring the Presence of Pro-inflammatory Cytokines||PDF 277 KB|
|应用文献||High Throughput Quantitation of Cytokine Biomarkers using LANCE Ultra TR-FRET Assays||PDF 1 MB|
|单页||LANCE Ultra Biomarker Detection Kits||PDF 585 KB|
|数据单表||LANCE Ultra human IL-6 Detection Kit||PDF 458 KB|
|产品手册||LANCE product listing||PDF 77 KB|
|应用文献||Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET||PDF 3 MB|