PerkinElmer
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Leptin (human) LANCE Ultra TR-FRET Detection Kit, 500 assay points

The LANCE® Ultra Human Leptin Detection Kit is designed for detection and quantitation of human leptin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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部件号 产品尺寸
TRF1225C 500 Assay Points
TRF1225M 10,000 Assay Points
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概览

The LANCE Ultra Human Leptin Detection Kit is designed for detection and quantitation of human leptin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

免责声明:  For research use only. Not for use in diagnostic procedures.

规格

检测目标 Leptin
检测目标类 Hormone
自动化兼容 Yes
实验类型 In vitro
产品品牌名称 LANCE Ultra
运输条件 蓝冰
Target Species Human
治疗领域 Metabolic
产品尺寸 500 Assay Points

资源,活动及更多信息

资源类型 文件名 文件格式
技术说明 Comparison of LANCE Ultra and HTRF Assays for Measuring the Presence of Pro-inflammatory Cytokines PDF  277 KB
单页 LANCE Ultra Biomarker Detection Kits PDF  585 KB
产品手册 LANCE product listing PDF  77 KB
应用文献 Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB