The LANCE® Ultra Human IgA Detection Kit is designed for detection and quantitation of human immunoglobulin A in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
|TRF1262C||500 Assay Points|
|TRF1262M||10,000 Assay Points|
The LANCE Ultra Human IgA Detection Kit is designed for detection and quantitation of human immunoglobulin A in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Immunoglobulin A (IgA) is a class of antibody mainly present in mucosal areas. IgA is essentially secreted in a dimeric form, which is maintained in place by the J-chain, a polypeptide of 15 kDa rich in cysteine residues. There are two isotypes of IgA: IgA1 is prevalent in the serum while IgA2 is prevalent in secretions. IgA is present in the gut, respiratory tract, urogenital tract, and in mucous secretions like saliva, tears, colostrum, vaginal fluid, and breast milk. IgA is relatively resistant to degradation and can remain active in extreme environments such as the digestive tract. IgA binds to pathogen, but is a poor activator of the complement. IgA deposition in the glomerulus is associated with IgA nephropathy.
免责声明: For research use only. Not for use in diagnostic procedures.
|检测方法||Time-Resolved Fluorescence (TRF), TR-FRET|
|产品尺寸||500 Assay Points|
|单页||LANCE Ultra Biomarker Detection Kits||PDF 585 KB|
|操作手册||LANCE Ultra human IgA Detection Kit||PDF 519 KB|
|产品手册||LANCE product listing||PDF 77 KB|
|应用文献||Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET||PDF 3 MB|