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IL-1RA (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human IL-1RA Detection Kit is designed for detection and quantitation of human interleukin-1RA in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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部件号 产品尺寸
TRF1358C 500 Assay Points
TRF1358M 10,000 Assay Points
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概览

The LANCE® Ultra Human IL-1RA Detection Kit is designed for detection and quantitation of human interleukin-1RA in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Interleukin-1 Receptor Antagonist (IL1RA) is a member of the interleukin-1 cytokine family. Immune, epithelial, and adipose cells secrete IL1RA which will bind to the interleukin-1 receptor and inhibit pro-inflammatory signaling through IL-1α and IL-1β. Studies have shown that IL1RA plays a key role in suppressing the pro-inflammatory effects of IL-1 in diseases like rheumatoid arthritis. Additionally, it has been observed that IL1RA may play an important role in diseases like osteoporosis where IL1RA levels are suppressed compared to controls. Furthermore, it has been observed that IL-1 cytokines are abundantly present at tumor sites and the addition of IL1RA can suppress growth of IL-1 producing tumors.

免责声明:  For research use only. Not for use in diagnostic procedures.

规格

检测目标 IL-1RA
检测目标类 Cytokine
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
实验类型 In vitro
产品品牌名称 LANCE Ultra
运输条件 蓝冰
Target Species Human
治疗领域 Inflammation
产品尺寸 10,000 Assay Points

资源,活动及更多信息

资源类型 文件名 文件格式
单页 LANCE Ultra Biomarker Detection Kits PDF  585 KB
操作手册 LANCE Ultra Human IL-1RA Detection Kit TRF1358 PDF  480 KB
产品手册 LANCE product listing PDF  77 KB
应用文献 Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB