PerkinElmer
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p53 (human) LANCE Ultra TR-FRET Detection Kit, 10,000 assay points

The LANCE® Ultra p53 Detection Kit is designed for detection and quantitation of human p53 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
TRF1388C
500 Assay Points
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TRF1388M
10,000 Assay Points
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詳情 信息

The LANCE® Ultra Human p53 Detection Kit is designed for detection and quantitation of p53 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Tumor protein p53, also known as p53, is a well conserved phosphoprotein composed of 390 amino acids. The p53 protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The p53 protein plays a major role in cellular response to DNA damage and other genomic aberrations. The loss of the p53 supressor function through mutations or inactivation of its pathway is associated with most if not all human cancers. Activation of p53 can lead to cell cycle arrest, DNA repair or apoptosis. p53 is phosphorylated at multiple sites by several different protein kinases. This kit quantifies the total amount of p53 in human serum, cell culture supernatants, and cell lysates.

规格

检测目标 p53
检测目标类 Protein
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
产品品牌名称 LANCE Ultra
运输条件 蓝冰
Target Species Human
治疗领域 Cancer
产品尺寸 10,000 Assay Points