The LANCE® Ultra p53 Detection Kit is designed for detection and quantitation of human p53 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
|TRF1388C||500 Assay Points|
|TRF1388M||10,000 Assay Points|
The LANCE® Ultra Human p53 Detection Kit is designed for detection and quantitation of p53 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Tumor protein p53, also known as p53, is a well conserved phosphoprotein composed of 390 amino acids. The p53 protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The p53 protein plays a major role in cellular response to DNA damage and other genomic aberrations. The loss of the p53 supressor function through mutations or inactivation of its pathway is associated with most if not all human cancers. Activation of p53 can lead to cell cycle arrest, DNA repair or apoptosis. p53 is phosphorylated at multiple sites by several different protein kinases. This kit quantifies the total amount of p53 in human serum, cell culture supernatants, and cell lysates.
免责声明: For research use only. Not for use in diagnostic procedures.
|检测方法||Time-Resolved Fluorescence (TRF), TR-FRET|
|产品尺寸||10,000 Assay Points|
|单页||LANCE Ultra Biomarker Detection Kits||PDF 585 KB|
|产品手册||LANCE product listing||PDF 82 KB|
|操作手册||Manual: LANCE Ultra human p53 Detection Kit||PDF 384 KB|
|应用文献||Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET||PDF 3 MB|