PerkinElmer
check quantity

Phospho-SMAD3 (Ser423/425) LANCE Ultra TR-FRET Cellular Detection Kit, 500 Assay Points

LANCE Ultra phospho-SMAD3 (Ser423/425) kits are designed for the detection of SMAD3 (phosphorylated at residue S423/S425) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

项目已成功添加到购物车

For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
TRF4014C
500 Assay Points
更多
TRF4014M
10,000 Assay Points
更多
立即购买

请输入有效的数量

请登录后再添加至收藏夹

购物车为空

詳情 信息

Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4014S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

规格

分析途径 TGFß/SMAD
检测目标 SMAD3
检测目标类 Phosphoprotein
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
产品品牌名称 LANCE Ultra
运输条件 蓝冰
Target Species Human
产品尺寸 500 Assay Points