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Phospho-STAT5 (Tyr694/699) LANCE Ultra TR-FRET Cellular Detection Kit, 10,000 Assay Points

LANCE Ultra phospho-STAT5 (Tyr694/699) kits are designed for the detection of STAT5 (phosphorylated at Y694/Y699) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
TRF4009C
500 Assay Points
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TRF4009M
10,000 Assay Points
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详情 信息

Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4009S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

STAT5 is a transcriptional activator which is also involved in intracellular signaling. Activation of STAT5 occurs upon phosphorylation at Tyr694. In some cancers STAT5 is shown to be constitutively activated. Activation can be triggered by a number of ligands including cytokines. STAT5 refers to two proteins with high sequence homology, STAT5A and STAT5B.

规格

分析途径 JAK/STAT
检测目标 STAT5
检测目标类 Protein
自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
运输条件 蓝冰
Target Species Human
产品尺寸 10,000 Assay Points