PerkinElmer
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IgE (mouse) isotyping AlphaLISA Detection Kit, 500 Assay Points

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgE antibodies in different sample matrices, including cell culture media. The IgE subtype plays a role in allergic reactions, parasitic infections and hypersensitivity reactions.

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部件号 产品尺寸
AL526HV 100 assay points
AL526C 500 assay points
AL526F 5,000 assay points
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概览

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. IgE has two main immune functions, it is the main driver in the immune resonse to parasites and it also is the main cause of allergic reactions. IgE is typically the least abundant immunoglobulin in serum but is capable of triggering the most powerful inflammation reations of all the isotypes. Typically mouse serum and plasma samples contain less than 75 ng/mL of IgE.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

免责声明:  For research use only. Not for use in diagnostic procedures.

规格

检测目标 IgE
检测目标类 Antibody
自动化兼容 Yes
检测方法 Alpha
实验类型 In vitro
产品品牌名称 AlphaLISA
运输条件 蓝冰
Target Species Mouse
治疗领域 Biologics/Bioprocess
产品尺寸 500 assay points

资源,活动及更多信息

资源类型 文件名 文件格式
产品手册 Alpha product listing PDF  124 KB
海报 AlphaLISA Isotyping Automation Poster PDF  767 KB
数据单表 Manual AlphaLISA Mouse IgE Isotyping AL526 PDF  274 KB
活动 Society of Laboratory Automation & Screening (SLAS)