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AlphaScreen DIG (Digoxin/Digoxigenin) Detection Kit, 50,000 assay points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-Dig (digoxin) conjugated AlphaScreen Acceptor beads. 

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部件号 产品尺寸
6760604C 500 assay points
6760604M 10,000 assay points
6760604R 50,000 assay points
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概览

This kit contains enough reagents to run 50,000 wells in 384-well format, using a 25 µL reaction volume.

This bead kit can be used for many applications, including:

  • Protein-protein interaction assays
  • Protein-peptide interaction assays
  • DNA-protein interaction assays
  • RNA-protein interaction assays
  • Dig tagged protein detection and quantitation
  • Protein expression
  • Binding studies

In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is Dig-tagged, and associates with the AlphaScreen anti-Dig Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated Dig probe provided in the kit.

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym ""Alpha"" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

免责声明:  For research use only. Not for use in diagnostic procedures.

规格

抗体偶联物 Streptavidin/Anti-DIG
自动化兼容 Yes
产品品牌名称 AlphaScreen
运输条件 蓝冰
产品尺寸 50,000 assay points

工具

资源,活动及更多信息

资源类型 文件名 文件格式
指南 Alpha Protein-Protein Interaction Quick Start Guide PDF  380 KB
产品手册 Alpha Technology Solutions PDF  4 MB
产品手册 Alpha product listing PDF  124 KB
指南 User's Guide To Alpha Assays Protein:Protein Interactions PDF  3 MB
活动 Society of Laboratory Automation & Screening (SLAS)