PerkinElmer
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AlphaScreen HA (Hemagglutinin) Detection Kit, 50,000 assay points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-HA (hemagglutinin) conjugated AlphaScreen Acceptor beads. 

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
6760612C
500 assay points
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6760612M
10,000 assay points
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6760612R
50,000 assay points
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詳情 信息

This kit contains enough reagents to run 50,000 wells in 384-well format, using a 25 µL reaction volume.

This bead kit can be used for many applications, including:

  • Protein-protein interaction assays
  • Protein-peptide interaction assays
  • HA-tagged protein detection and quantitation
  • Protein expression
  • Binding studies

In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is HA-tagged, and associates with the AlphaScreen anti-HA Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated HA probe provided in the kit.

规格

抗体偶联物 Streptavidin/Anti-HA
自动化兼容 Yes
产品品牌名称 AlphaScreen
运输条件 蓝冰
产品尺寸 50,000 assay points