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Operetta CLS 高内涵系统

Operetta CLS™ 系统帮助您探索常规实验与复杂创新实验中的生物学信息。通过一系列创新技术的有机结合,Operetta CLS™ 同时满足精细细胞细节成像对高速、高灵敏度、高分辨率检测多方面的需求。同时,凭借功能强大的Harmony 4.5版软件,Operetta CLS™ 可帮助您发现并定量细微的细胞表型改变。

部件号 HH16000000
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详情 信息

Operetta CLS™ 系统同时兼具高速、高灵敏度的数据采集能力,以及广受赞誉的功能强大,界面友好的数据分析解读功能。为了进一步助力您的研究工作,我们提供了从全套高内涵筛选的解决方案,从高内涵仪器到成像使用标准多孔板到自动化液体工作站以及后续丰富的信息学数据处理解决方案。Perkinelmer为你提供一站式的无缝连接服务,帮助您搭建全面专业高内涵筛选平台。完善的平台搭建服务与界面友好且功能强大的高内涵分析软件harmony ,帮您扫除高内涵筛选路上的后顾之忧。与您一路同行,快速高效的开始您的高内涵筛选工作。

多项专为高内涵筛选研发的先进技术的有机组合

Operetta CLS高内涵筛选系统以全新设计的高灵敏的光路为核心,确保最高效的样品信息的激发和检测。

  • 首开先河的高能、长寿命8色高内涵固态LED光源提供最优化、最可靠地荧光信号采集和数据采集。
  • 专利全自动水镜系统同时满足了细胞图像采集在高分辨率、高速以及光毒性最小的需求。
  • 转盘共聚焦提供了快速、低光毒性的共聚焦成像,在获得共聚焦质量图像的同时,提供了活细胞在线实时检测以及三维扫描检测功能。
  • 大靶免sCMOS相机提供了低噪声、宽动态范围、高分辨率的图像检测,为低光毒性、高灵敏度、定量检测提供了绝佳的检测方案。
  • 用户友好的编码型滤光片,提供了灵活、特异性高、且易于操作的成像体验。
  • 简单且功能强大的Harmony 4.5高内涵软件成像分析软件提供了符合生物学使用习惯、逻辑清晰、功能强大的实验设置、图像采集、图像分析以及数据存储和解读。

为您实验室量身定做的高内涵解决方案:

Operetta CLS™ 满足您实验中的多种实验需求。您可根据您的实验需求定制不同模块的高内涵系统。推荐配置包括:

Operetta CLS™ Quattro

  • 四色LED以及宽场荧光,Operetta CLS基础配置满足您实验室日常实验所需要的灵敏度和分辨率,同时为您提供了保留了丰富的升级空间。

Operetta CLS™ FLEX

  • 八色LED,共聚焦及宽场荧光成像,FLEX 配置提供全面的、灵活的、特异性极高的荧光激发、荧光检测配置,同时该型号也提供更加高端的性能升级空间。

Operetta CLS™ LIVE

  • 在FLEX型号基础上,配置活细胞气体控制与温度控制以及低光毒性、高分辨率、高荧光亮度的水镜头系统,该系统是低光毒性、高灵敏度的活细胞成像实验的理想选择。

规格

21 CFR Part 11兼容 No
自动化兼容 Yes
检测方法 Transmission, Fluorescence
高度 45.0 cm
显像模式 High Content
接口 Ethernet
长度 98.0 cm
光源 LED
便携式 No
产品品牌名称 Operetta CLS
保修 Yes
宽度 66.0 cm
资源,活动及更多信息
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网络研讨会

Image-Based Screening for Precision Medicine and Immunotherapy

Discover how image-based screening can be performed directly in primary material, enabling more clinically translatable drug screening, and ultimately, functional precision medicine.

网络研讨会

产品手册

HCS Operetta CLS 应用案例

高内涵应用案例

PDF 6 MB
Operetta CLS

高内涵成像筛选系统Operetta CLS

PDF 1 MB
With 3D Cell Cultures Your Science Comes Alive

Download our brochure to learn how our solutions help you to grow, detect, and analyze 3D cell

PDF 4 MB
药物筛选完全解决方案

从高通量到高内涵药物筛选完全解决方案

PDF 1 MB

应用文献

Analyzing ERK Signal Transduction in Live Cells Using a FRET-Based Biosensor

Extracellular signal-regulated kinase (ERK) is a key component in the regulation of embryogenesis, cell differentiation, cell proliferation, and cell death. The ERK signaling pathway is altered in various cancer types and is frequently investigated as a target for therapeutic intervention. This application note describes how a live cell FRET assay to study ERK signaling was performed on the Operetta CLS™ high-content analysis system. The optimized design of the FRET-based biosensor, the high-quality imaging of the Operetta CLS system and the easy-to-use image analysis tools of the Harmony® software contribute to the robustness of the high-content assay.

PDF 1 MB
Automated Single Cell Tracking using the Operetta System: Analyzing Chemokinesis of Cancer Cells

In this application note, we describe a high-content screening application for analyzing the migration of non-small cell lung cancer cells in a live cell assay. Using the Operetta® high-content imaging system and digital phase contrast imaging, we tracked migrating cancer cells using automated single cell tracking in the Harmony® high-content imaging and analysis software.

PDF 1 MB
Phenotypic Analysis of Hypertrophy in Human iPSC-Derived Cardiomyocytes

Learn how a phenotypic screening assay to study time-dependent effects of endothelin-1-induced hypertrophy was set up using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. Learn how: The Opera Phenix system has been applied in the field of neurodegenerative diseases. In this assay, the Opera Phenix system is 4 times faster than the previous Opera® system. Primary neuron morphology is analyzed in a straightforward approach using Harmony software. Careful assay optimization can increase throughput, and minimize the data burden, without compromising assay performance.

PDF 1 MB

技术说明

3D Volumetric Analysis of Luminal Spaces Inside Cysts or Organoids

High-content assays using 3D objects such as cysts or organoids can be challenging from the perspectives of both image acquisition and image analysis. In this technical note we describe how to image and analyze epithelial Madin-Darby canine kidney (MDCK) cysts in 3D on the Operetta CLS™ high-content analysis system.

PDF 1 MB
How to Perform Successful Long Term Live Cell Imaging in a High-Content Analysis System

Download our technical note to find out how you can overcome some of the challenges associated with long-term live cell imaging. Learn how you can perform successful five-day live cell imaging on Operetta CLS™ and Opera Phenix™ high-content systems, avoid phototoxicity with gentle digital phase contrast imaging, and analyze cell growth and morphology on a single cell level without fluorescence staining.

PDF 3 MB
The Benefits of Automated Water Immersion Lenses for High-Content Screening

Balancing the key factors in HCS imaging - sensitivity, resolution and speed - can be challenging since they cannot be optimized independently: changing one impacts the others. Nevertheless, there is a way to overcome some of the obstacles and here we explain why the choice of the objective lens is critical.

PDF 1 MB

操作手册

Five Top Tips for a Successful High-Content Screening Assay with a 3D Cell Model System

Whether you’re familiar with high-content screening, or a newcomer, you’ll need the right tools and strategies to overcome the challenges of using complex 3D cell models in such an assay. For example, growing consistent, reproducible 3D cultures can be problematic and imaging large, thick cell samples can be challenging, while managing the huge volumes of data generated is perhaps the most demanding aspect of all. In this article, we provide our top tips for running a successful high-content screening assay using a 3D cell model. Learn how you can: Generate uniform 3D cell models, Get the best quality images, Minimize imaging time and volume of data, Get deeper insights from your 3D cell model and Avoid unnecessary data transfer steps.

PDF 1 MB

文章

5 Top Tips for a Successful High-content Screening Assay with a 3D Cell Model System

Whether you’re familiar with high-content screening and are looking to exploit the increased physiological relevance of complex 3D cell models, or you want to take your analysis of 3D cell models to the next level, migrating from simple plate-reader assays to a high-content approach, you’ll need the right tools and strategies to overcome the challenges these models present.

PDF 1 MB

案例分析

A Workflow to Characterize and Benchmark Human Induced Pluripotent Stem Cells

Human induced pluripotent stem cells (iPSCs) offer tremendous opportunities for disease modeling and discovery of novel therapeutics. The UK-based Human Induced Pluripotent Stem Cell Initiative (HipSci) aims to advance iPSC technology and pave the way to discovering how genomic variation impacts cellular phenotype by offering the scientific community access to a vast panel of cell lines with thorough characterization and data analysis tools. This case study details a phenotypic screen used to characterize human iPSCs on diverse extracellular matrix substrates, and a method for the capture of specific phenotypes emerging upon cell-to-cell contact.

PDF 1 MB
High-Content Analysis of Drug-Induced Oligodendrocyte Differentiation Promoting Remyelination in Multiple Sclerosis

One of the greatest challenges in multiple sclerosis (MS) therapy is the halting or reversal of the failure of remyelination in the brain in order to reverse disabilities in MS patients. This case study highlights the recent work of Dr. Paul Tesar and colleagues at the Case Western Reserve University School of Medicine, which could potentially lead to such novel treatments, as it aims to control the function of stem cells in the body and thereby to help the body repair itself.

PDF 3 MB
High-Content Analysis: Advancing Your Knowledge to Help Combat Infectious Disease

Infectious diseases remain a major burden to human health. The increased globalization of modern society that facilitates the spread of infectious diseases, and phenomena such as anti-microbial resistance, underscore the importance of the development of new preventative and therapeutic approaches. In our e-book, learn how high-content imaging and analysis plays a significant role in infectious disease research.

PDF 4 MB