PerkinElmer
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Protein A PVT 500 MG

Protein A-coated SPA beads, for capturing antibodies in proximity-based radiometric scintillation assays.

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部件号
RPNQ0019
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概览

SPA Scintillation beads are microspheres containing scintillant which emit light in the blue region of the visible spectrum. As a result, these beads are ideally suited to use with photomultiplier tube (PMT) counters such as the MicroBeta2 or TopCount.

Two types of core SPA Scintillation bead are available - yttrium silicate (YSi) and Polyvinyltoluene (PVT). PVT beads are plastic, larger in size, and stay in suspension longer than the crystalline YSi beads.

Scintillation proximity assay (SPA) is a homogeneous and versatile technology for the rapid and sensitive assay of a wide range of biological processes, including applications using enzyme and receptor targets, radioimmunoassays, and molecular interactions. When 3H, 14C, 33P, and 125I radioisotopes decay, they release β-particles (or Auger electrons, in the case of 125I). The distance these particles travel through an aqueous solution is dependent on the energy of the particle. If a radioactive molecule is held in close enough proximity to a SPA Scintillation Bead or a SPA Imaging Bead, the decay particles stimulate the scintillant within the bead to emit light, which is then detected in a PMT-based scintillation counter or on a CCD-based imager, respectively. However, if the radioactive molecule does not associate with the SPA bead, the decay particles will not have sufficient energy to reach the bead and no light will be emitted. This discrimination of binding by proximity means that no physical separation of bound and free radiochemical is required.

规格

自动化兼容 Yes
珠型或核心珠型 PVT
涂层处理 Protein A
检测方法 Radiometric
产品品牌名称 SPA Scintillation Beads
运输条件 环境
产品尺寸 500 mg

资源,活动及更多信息

资源类型 文件名 文件格式
应用文献 Assay Development Guide to Protein:Protein Studies PDF  951 KB
应用文献 The Application of SPA Technology to Study Protein: DNA Interactions PDF  997 KB
应用文献 The Application of SPA to SH2 and SH3 Domain Binding to Specific Peptide Sequences PDF  1 MB