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[Methyl-14C] Methylated, Protein Molecular Weight Markers, 5µCi (185kBq)

14C-labeled markers are for use in radiometric applications. The eletrophoretic mobility of methylated molecular weight markers is identical to the unmodified individual protein.

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NEC811001UC
1 µCi
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NEC811005UC
5 µCi
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详情 信息

14C-labeled protein molecular weight markers for gel electrophoresis and autoradiography. Gel electophoresis is a widely employed technique for determining the molecular weight of proteins. The mobility of a protein on polyacrylamide gels containing sodium dodecylsulfate (SDS) is primarily a function of the size of the macromolecule. 0.02 µCi of each marker is sufficient for overnight detection by autogradiography.

  • Phosphorylase B: 97,400 Da
  • Albumin (BSA): 69,000 Da
  • Gammaglobulin subunit 1: 55,400 Da
  • Gammaglobulin subunit 2: 30,000 Da
  • Ovalbumin: 46,000 Da
  • Cytochrome C: 12,300 Da

Sample preparation for electrophoresis. Just prior to use:

  1. Make sure the solution is homogeneous. Some precipitation of the SDS is likely to occur upon thawing. It can be brought back to solution by gently warming the solution to 37 °C.
  2. Add 2-mercaptoethanol and SDS to your sample loading buffer (5% by volume) in a snap cap polypropylene tube and place in a boiling water bath for 3-5 minutes.

规格

浓度 0.005 mCi/mL
检测方法 Radiometric
标签位置 Specifically Labeled
放射性同位素 C-14
放射性核素 14C
运输条件 干冰
特殊订购信息 This is a radioactive product - shipping address must have a license to receive radioactive materials.
产品尺寸 5 µCi
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产品手册

Radiometric Reagents Guide 2010-2011

For over 50 years PerkinElmer has been a leading supplier of radiochemicals, liquid scintillation cocktails, vials and nuclear counting detection instruments. Today is no different. We have always been committed to providing you products for all of your radiometric needs and we are still committed today.

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指南

Carbon-14 Handling Precautions

This document contains general information designed to provide a basic understanding of radiation safety. While we believe the information to be accurate, regulatory requirements may change and information contained herein is not tailored to individual needs.

PDF 102 KB
Chromium-51 Handling Precautions

This document contains general information designed to provide a basic understanding of radiation safety. While we believe the information to be accurate, regulatory requirements may change and information contained herein is not tailored to individual needs.

PDF 107 KB