PerkinElmer
check quantity

Purinergic P2Y11, Human Recombinant, 1321N1 AequoZen Frozen cells

Our frozen, irradiated AequoZen cells express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light. No cell culture is required prior to testing, for these frozen cells.

部件号 ES-293-AF
立即购买

请输入有效的数量

请登录后再添加至收藏夹

购物车为空

项目已成功添加到购物车

This product contains living cells. Some AequoZen™ FroZen Cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.

详情 信息

Frozen Cells validated for calcium testing: Purinergic P2Y11 receptor, human recombinant, in 1321N1 host cell. We provide one vial of frozen cells (10 million cells/vial). Some of our Frozen cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information. You are authorized to utilize these frozen cell preparations one time only. Any attempt to transfer, re-use, or propagate these cells is expressly unauthorized and a violation of the product terms and conditions of sale.

Features:

  • Pre-validated batches of ready-to-use cells for calcium testing
  • Optimized to alleviate biological variability
  • Step-by-Step protocols for culture-free functional testing on our validated cells
  • Affordable access to small aliquots of cells (minimum size: 10 million cells/vial)
  • Ready-to-use cells for Profiling, Pharmacology or Screening applications
  • Up-scaling and customized developments

The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

The frozen cells approach to functional testing consists in the dissociation of cell culture from testing. That means that we will culture the cells and freeze them with an optimized protocol to stop all replication. These cells are then stored in liquid nitrogen or simply in a -80°C freezer, thawed and used directly in a functional, cellular GPCR test with previously validated performance.

规格

检测目标类 GPCR
检测目标类型 Cell line
检测验证 Calcium Luminescence
G-Alpha天然受体 Gq/G11
宿主细胞 1321N1
产品品牌名称 AequoZen
受体类型 Purinergic P2Y
第二信使释放 Calcium flux
运输条件 干冰
治疗领域 Inflammation, Metabolic
产品尺寸 10 million cells
资源,活动及更多信息
  • 所有

产品手册

EnSpire Multimode Plate Reader with Label-free Technology Brochure

Now you can characterize cellular signaling mechanisms and better understand the complexity of multiple signaling pathways more efficiently. Only the EnSpire® Multimode Plate Reader offers established Corning® Epic® label-free technology in addition to labeled technologies all within one flexible benchtop reader. This exclusive cellular and biochemical label-free platform, in conjunction with labeled technologies, provides a unique orthogonal approach, enabling you to make better decisions faster.

PDF 4 MB

单页

Aequorin FLIPR

Aequorin and Photina cell lines – the alternative calcium flux assay. PDL-coated microplates improve your performance

PDF 1 MB

应用文献

AequoScreen aequorin-encoding Plasmids for performing aequorin luminescent assay starting from non-aequorin cell lines

Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.

PDF 607 KB
Aequorin bioluminescence assays on MicroBeta2 LumiJET for studying Ca2+-coupled GPCRs and ion channels

Aequorin-based Ca2+ assays represent a new paradigm in drug discovery research for cell-based assays for Ca2+-coupled GPCRs and ion channels. In the aequorin assay, cells co-expressing apoaequorin and the target receptor are first incubated with the co-factor coelenterazinein order to reconstitute the activea equorin enzyme.

PDF 152 KB