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AlphaScreen Histidine (Nickel Chelate) Detection Kit, 50,000 assay points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and nickel chelate (Ni-NTA) AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.




部件号 产品尺寸
6760619C 500 assay points
6760619M 10,000 assay points
6760619R 50,000 assay points



This bead kit can be used for many applications, including:

  • Protein-protein interaction assays
  • Protein-peptide interaction assays
  • His-tagged protein detection and quantitation
  • Protein expression
  • Binding studies

In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is His-tagged, and associates with the AlphaScreen nickel chelate Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated His peptide probe provided in the kit.

免责声明:  For research use only. Not for use in diagnostic procedures.


抗体偶联物 Streptavidin/Nickel chelate
自动化兼容 Yes
产品品牌名称 AlphaScreen
运输条件 蓝冰
产品尺寸 50,000 assay points


资源类型 文件名 文件格式
指南 Alpha Protein-Protein Interaction Quick Start Guide PDF  380 KB
产品手册 Alpha Technology Solutions PDF  4 MB
产品手册 Alpha product listing PDF  128 KB
指南 User's Guide To Alpha Assays Protein:Protein Interactions PDF  3 MB
活动 Society of Laboratory Automation & Screening (SLAS)