Europium labeling chelate for creating your own Europium-labeled biomolecules for use in LANCE TR-FRET assays. The iodoacetamido-activated chelate reacts primarily with free sulfhydryl groups, such as cysteines.
LANCE® Eu-W1284 iodoacetamido Chelate is optimized for the labeling of proteins and peptides at least one sulfhydryl group. The labeled compound can be used in homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) assays (LANCE® assays).
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC)]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
|检测方法||Time-Resolved Fluorescence (TRF), TR-FRET|
Iodoacetamido-activated (IAA-activated) Eu-W1284 chelate is optimized for labeling proteins and peptides containing at least one sulfhydryl group.