The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-eIF4E (Ser209) in a multiplexed assay.
|TBSU-PEIF4-A-HV||100 Assay Points|
|TBSU-PEIF4-A500||500 Assay Points|
|TBSU-PEIF4-A10K||10,000 Assay Points|
|TBSU-PEIF4-A50K||50,000 Assay Points|
Alpha SureFire assays are cellular assays used to detect and quantitate phosphoproteins for cell signaling, pathway analysis, and cellular kinase studies using homogeneous (no wash steps; no separation steps) Alpha technology. Terbium SureFire assays enable multiplexing for detection of two phosphoproteins in a single well, when used in conjunction with any AlphaLISA SureFire Ultra kit. This multiplex measurement is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (Terbium: 545 nm, Europium: 615 nm). The two distinct Alpha Acceptor beads report their binding to distinct antigens through their association with specific assay antibodies.
The Terbium “Alpha 545 Acceptor Beads” in the kit are conjugated with a “CaptSure2” agent, which binds a specific CaptSure2 tag on one of the assay antibodies. In combination with the biotinylated antibody provided, the 545 nm signal generated will report levels of the phosphoprotein of interest (Target 2). This kit is to be used in conjunction with the reagents in an AlphaLISA SureFire Ultra kit, which will report that target-of-interest by Europium emission at 615 nm (Target 1).
*These assays utilize the bead-based Alpha Technology, and requires an Alpha Technology-compatible plate reader capable of reading dual emission wavelengths. Refer to the “AlphaPlex Quick Start Guide” and the “AlphaPlex Assay Development Guide” to find guidance about filters and mirrors selection, instrument protocol and channels crosstalk correction.
免责声明: For research use only. Not for use in diagnostic procedures. Please note this kit does not contain all buffers necessary to run the assay, and must be used with an AlphaLISA SureFire Ultra assay kit to obtain all required reagents.
|产品品牌名称||Alpha SureFire Ultra Multiplex|
|产品尺寸||100 Assay Points|
|应用文献||Alpha Terbium SureFire Ultra Multiplex: Simultaneous Dual Phosphoprotein Target Analysis||PDF 1 MB|
|产品手册||Alpha product listing||PDF 128 KB|
|指南||AlphaPlex Assay Development Guide 2015||PDF 1 MB|
|指南||AlphaPlex Quick Start Guide||PDF 431 KB|
|技术说明||Guide for Reading SureFire Multiplexed assays on the EnVision||PDF 2 MB|
|操作手册||Protocol for Alpha Terbium SureFire Ultra High Volume Kit (HV) Assays||PDF 1 MB|
|技术说明||Specificity and control lysate details for TBSU peIF4E kit||PDF 127 KB|