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LANCE Ultra ULight-Glycogen Synthase (Ser641/pSer657) Peptide, 5 nmoles

Glycogen synthase peptide (Sequence: PASVPPSPSLSRHSSPHQ(pS)ED) labeled with ULight™ dye. Synthetic peptide containing residues surrounding Ser641 and Ser657 of human Muscle Glycogen Synthase. This peptide is pre-phosphorylated on Ser657. Target phosphorylation site: Ser641. This peptide substrate can be phosphorylated by a variety of kinases including GSK3α and GSK3β.




部件号 产品尺寸
TRF0131-D 0.5 nmol
TRF0131-M 5 nmol



LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:

  • Test conditions: Rapid assay setup to move straight into optimized assay performance

  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing

  • Phospho-motifs: Substrate sequences are provided

  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For ULight substrates:

  • 0.5 nmoles = 1,000 assay points

  • 5 nmoles = 10,000 assay points

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.


自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
实验类型 In vitro
荧光基团 ULight
分子修饰 Phosphorylation
产品品牌名称 LANCE Ultra
运输条件 干冰
产品尺寸 5 nmol


资源类型 文件名 文件格式
应用文献 Comparison of LANCE Ultra TR-FRET to PerkinElmer's Classical LANCE TR-FRET Platform for Kinase Applications PDF  805 KB
应用文献 Improved Stability of the LANCE Ultra Signal in Kinase Assays PDF  206 KB
产品手册 LANCE product listing PDF  82 KB
技术说明 Technical note: GSK3ß Kinase Assay PDF  1022 KB