PerkinElmer
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LANCE Ultra ULight™-DNA Topoisomerase 2-alpha (Thr1342) Peptide, 5 nmoles

Synthetic peptide containing residues surrounding Thr1342 of human DNA Topoisomerase 2-alpha; phosphorylation site: Thr1342. The ULight-DNA Topoisomerase 2-alpha (Thr1342) peptide can be phosphorylated by PLK3, CK2α and other kinases. Phosphorylation motif: DEKTDDE

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TRF0130-D
0.5 nmol
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TRF0130-M
5 nmol
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详情 信息

LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:

  • Test conditions: Rapid assay setup to move straight into optimized assay performance
  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing
  • Phospho-motifs: Substrate sequences are provided
  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For ULight substrates:

  • 0.5 nmoles = 1,000 assay points
  • 5 nmoles = 10,000 assay points

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.

规格

自动化兼容 Yes
检测方法 Time-Resolved Fluorescence (TRF), TR-FRET
实验类型 In vitro
荧光基团 ULight
分子修饰 Phosphorylation
产品品牌名称 LANCE Ultra
运输条件 干冰
产品尺寸 5 nmol
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应用文献

Improved Stability of the LANCE Ultra Signal in Kinase Assays

LANCE® Ultra is a high through putscreening (HTS) technology platform optimized for homogeneous time-resolved fluorescence resonance energy transfer (TRFRET) kinase assays. The LANCEUltra donor dye is a Europium(Eu) chelate, and the acceptor dye is a proprietary small-molecular weight molecule called ULight™, a fluorescent dye with a red-shifted light emission.

PDF 206 KB

技术说明

CK2a1/b Kinase Assay ULight™ - DNA Topoisomerase 2-alpha (Thr1342) Peptide Europium-anti-phospho-DNA Topoisomerase 2-alpha (Thr1342) Antibody

LANCE Ultra time-resolved fluorescence resonance energy transfer (TR-FRET) assays use a proprietary europium chelate donor dye with ULight, an innovative small molecular weight acceptor dye with a red-shifted fluorescent emission.

PDF 939 KB
PLK3 Kinase Assay ULight™ - DNA Topoisomerase 2-alpha (Thr1342) Peptide Europium-anti-phospho-DNA Topoisomerase 2-alpha (Thr1342) Antibody

LANCE® Ultra time-resolved fluorescence resonance energy transfer (TR-FRET) assays use a proprietary europium chelatedonor dye, W-1024 (Eu), with ULight, an innovative small molecular weight acceptor dye with a red-shifted fluorescent emission.

PDF 1011 KB