PerkinElmer
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AlphaScreen Unconjugated Donor Beads, 1mg

Alpha Unconjugated Donor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own Alpha Donor beads for use in Alpha no-wash assays.

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For research use only. Not for use in diagnostic procedures.

部件号
产品尺寸
6762013
1 mg
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6762011
5 mg
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6762012
50 mg
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详情 信息

These beads can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:

  • Analyte detection assays
  • Protein-protein interaction assays
  • Protein-DNA interaction assays
  • Protein-RNA interaction assays
  • Protein-small molecule interaction assays
  • Protein detection assays
  • Antibody detection assays
  • Antibody binding assays
  • Enzymatic assays
  • Immunogenicity studies

In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.

规格

抗体偶联物 Unconjugated
自动化兼容 Yes
珠型或核心珠型 Alpha Donor
产品品牌名称 Alpha
运输条件 蓝冰
产品尺寸 1 mg
资源,活动及更多信息
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产品手册

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 4 MB
Handle Large Biomolecular Interactions With Ease

The interactions and bindingof proteins are implicated in a large number of biological processes. The needfor an efficient, highly sensitive assay to study large protein interactions is increasingly important. Alpha Technology is a highly flexible, homogeneous, no-wash assay ideal for the measurement of protein interactions and complexes as large as 200 nm in size

PDF 798 KB

应用文献

Immunogenicity Assessment Using the AlphaLISA Technology

Biological drug products often elicit an immune response in patients. Clinical consequences of the presence of anti-drug antibodies (ADA) can vary from mild to serious adverse events. Therefore, the presence of ADA is a major safety and efficacy concern and should be evaluated and correlated with any pharmacological or toxicological observations.

PDF 289 KB
Immunogenicity Assessment Using the AlphaLISA technology

Immunogenicity,Assessment Using the,AlphaLISA technology

PDF 292 KB

指南

Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.

PDF 380 KB
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

PDF 1 MB
User's Guide To Alpha Assays Protein:Protein Interactions

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.

PDF 3 MB