Uridine triphosphate, labeled on the alpha phosphate group with 35S, is typically used in reactions involving enzymes that will incorporate the ribonucleoside monophosphate (base, sugar, and alpha-labeled phosphate) into a chain of RNA. The advantage of 35S, when compared to other short-lived isotopes, is its relatively low signal (which contributes to a sharp band width), as well as longer shelf life.
Common applications include:
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