Uridine triphosphate, labeled on the alpha phosphate group with 35S.
Uridine triphosphate, labeled on the alpha phosphate group with 35S, is typically used in reactions involving enzymes that will incorporate the ribonucleoside monophosphate (base, sugar, and alpha-labeled phosphate) into a chain of RNA. The advantage of 35S, when compared to other short-lived isotopes, is its relatively low signal (which contributes to a sharp band width), as well as longer shelf life.
Common applications include:
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